Encyclopedia: Thrombin
Thrombin is a coagulation protein that has many effects in the coagulation cascade. It is a serine protease that converts soluble fibrinogen into insoluble strands of fibrin, as well as catalyzing many other coagulation-related reactions. The prothrombin gene is located on the eleventh chromosome . The molecular weight of prothrombin is approximately 72000mol-1; in contrast, the molecular weight of thrombin is 36000mol-1. Once activated, the catalytic domain of prothrombin is...
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How can I get ride off thrombin?
I am expressing a recombinant protein in E coli with a his tag. In order...
I am expressing a recombinant protein in E coli with a his tag. In order...
Perhaps the thrombin and recombinant protein molecules are sufficiently different to separate using column chromatography.
Asked by netto luis - 32 months ago
problem in thrombin digestion in GST tagged protein purification?
I have successfully over expressed my GST tagged protein in BL21 by using PQE30 vector...
I have successfully over expressed my GST tagged protein in BL21 by using PQE30 vector...
You need to trouble-shoot 1. Is your thrombin still active? 2. is your thrombin site available for cleavage? I usually elute the purified protein (with glutathione), dialyze, cleave, then repass over the GST...
Asked by dimple k - 7 months ago
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