Dna Probe Overview

Encyclopedia: Hybridization Probe

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In molecular biology, a hybridization probe is a fragment of DNA of variable length , which is used to detect in DNA or RNA samples the presence of nucleotide sequences that are complementary to the sequence in the probe. The probe thereby hybridizes to single-stranded nucleic acid whose base sequence allows probe-target base pairing due to complementarity between the probe and target. The labeled probe is first denatured into single...


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Question and Answer

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how to know the base sequence of DNA used in DNA probe?
in DNA probe, short single stranded dna labelled with radioactive is mixed with the double...

The probe is just the complimentary sequence of the gene, so that it will bind specifically to your gene of interest. This is only done when the sequence of...

Asked by farfalle - 22 months ago

1) Why does DNA migrate in the gel electrophoresis segment?........2) What is a DNA probe?...?...
3) WHAT ARE SOME OTHER USES FOR THIS DNA FINGERPRINTING TECHNIQUE?..

1. DNA has a negative charge. Thus when a charge is applied to the gel the DNA will migrate through the gel towards the positive electrode. 2. A DNA probe...

Asked by sweetie - 8 months ago

Question & Answer

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  • Why do aliens want to probe you?

    Duh - just look at ALL THIS. Who wouldn't want to probe me? *joke*...

  • Do fraternal twins have the same dna?

    Only identical twins share the same DNA. And even their DNA isn't exactly identical. Studies show that there are very slight differences. In fact so slight that most DNA tests can't pick them up. Fraternal...

Forum Posts

OmgiliOMGILI

  • Digoxigenin as a label for DNA probes - 2 replies

    hi there, can anyone tell me any advantages of using digoxigenin as a label for DNA probes instead RNase A treatment removes much of the non-specifically bound probe Very sensitive...

    Nov 04, 2004

    www.omgili.com

  • DNA-probe labelling - 2 replies

    HI! I'm looking for a very short description (just the idea) of methods used to label DNA probes Most frequently are used labeled nucleotides during DNA amplification or 3 end labeling...

    Jul 20, 2004

    www.omgili.com

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